RNA

Part:BBa_K2715032:Design

Designed by: Daniel Partridge   Group: iGEM18_Nottingham   (2018-10-12)


Synthetic guide RNA targeting sequence 1 for tcdA promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This guide RNA sequence was designed using the CRISPR guide selection tool of the online resource Benchling.

Source

This is a 20 bp targeting region of a synthetic guide RNA designed to work with the Cas9 protein from S. pyrogenes. It was taken from the promoter region of toxin A in C. difficile